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RNPS1 antibody

KO/KD
Anti-RNPS1 antibody used in Western Blot (WB). GTX129789
Anti-RNPS1 antibody used in Western Blot (WB). GTX129789

Cat. No. GTX129789

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all RNPS1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Rat, Bovine, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human RNPS1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886089

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RNA binding protein with serine rich domain 1 , E5.1

Cellular Localization

Nucleus , Nucleus speckle , Cytoplasm

Background

This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. This protein contains many serine residues. Two splice variants have been found for this gene; both variants encode the same protein. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RNPS1 antibody used in Western Blot (WB). GTX129789

GTX129789 WB Image

RNPS1 antibody detects RNPS1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10 % SDS-PAGE, and blotted with RNPS1 antibody (GTX129789) diluted by 1:1000

Anti-RNPS1 antibody used in Western Blot (WB). GTX129789

GTX129789 WB Image

KO/KD

The data was published in the journal Genes (Basel) in 2014.PMID: 25333844

REFERENCE

Application Reference

Zhong X et al. Proc Natl Acad Sci U S A 2022; 119 (18) : e2200128119 RNPS1 inhibits excessive tumor necrosis factor/tumor necrosis factor receptor signaling to support hematopoiesis in mice.
Application : WB
Reactivity : Mouse
Hou S et al. Nucleic Acids Res 2019; 47 (15) : 8239-8254 XAB2 depletion induces intron retention in POLR2A to impair global transcription and promote cellular senescence.
Zhang H et al. Genes (Basel) 2014; 5 (4) : 982-1000 Sumoylation is Required for the Cytoplasmic Accumulation of a Subset of mRNAs.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169