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Orai1 antibody

Anti-Orai1 antibody used in Flow cytometry (FACS). GTX16613
Anti-Orai1 antibody used in Live cell imaging (LCI). GTX16613
Anti-Orai1 antibody used in Western Blot (WB). GTX16613
Anti-Orai1 antibody used in IHC (Paraffin sections) (IHC-P). GTX16613

Cat. No. GTX16613

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS, LCI

Reactivity

 Mouse, Rat
Package
50 μl ($639)
See all Orai1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
FACS Assay dependent
LCI Assay dependent
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Product Note

We do not recommend use of this product for Human samples.

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.65 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Peptide (C)KFLPLKRQAGQPS, corresponding to amino acid residues 200-212 (2nd  extracellular loop) of mouse Orai1 (Accession  : Q8BWG9).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887543

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ORAI calcium release-activated calcium modulator 1 , D730049H07Rik , Tmem142a , orai-1

Cellular Localization

Cell membrane

Database

Research Area

DATA IMAGES

Anti-Orai1 antibody used in Flow cytometry (FACS). GTX16613

GTX16613 FACS Image

FACS analysis of WEHI cells using GTX16613 Orai1 antibody.
Black : Unstained cell
Green : Cell staining with primary antibody
Antibody amount : 10 μg antibody / 0.5 x 10⁶  cells

Anti-Orai1 antibody used in Live cell imaging (LCI). GTX16613

GTX16613 LCI Image

Live cell imaging analysis of intact living RBL cells using GTX16613 Orai1 antibody.
Panel A : Primary antibody
Panel B : Merge of panel A with the live view of the cell
Dilution : 1:100

Anti-Orai1 antibody used in Western Blot (WB). GTX16613

GTX16613 WB Image

WB analysis of rat spleen (lanes 1 and4), rat pancreas (lanes 2 and 5), and mouse B-cell lymphoma (WEHI) (lanes 3 and 6) lysates using GTX16613 Orai1 antibody preincubated with or without immunogen peptide.
Dilution : 1:200

Anti-Orai1 antibody used in IHC (Paraffin sections) (IHC-P). GTX16613

GTX16613 IHC-P Image

IHC-P analysis of rat pancreas tissue using GTX16613 Orai1 antibody. Orai1 (brown) is expressed in acinar cells. Hematoxilin is used as the counterstain.
Dilution : 1:50

REFERENCE

Application Reference

Aysegul Durak et al. BMC Cardiovasc Disord 2022; 22 (1) : 147 STIM1-Orai1 interaction mediated calcium influx activation contributes to cardiac contractility of insulin-resistant rats.
Application : WB
Bohineust A et al. J Exp Med 2018; (Epub) Termination of T cell priming relies on a phase of unresponsiveness promoting disengagement from APCs and T cell division.
Application : FACS
Reactivity : Mouse
Wong JH et al. Sci Rep 2017; 7 (1) : 12813 Store-operated Ca2+ Entry Facilitates the Lipopolysaccharide-induced Cyclooxygenase-2 Expression in Gastric Cancer Cells.
Application : WB
Reactivity : Human
Ong HL et al. Sci Signal 2015; 8 (359) : ra3 STIM2 enhances receptor-stimulated Ca2 signaling by promoting recruitment of STIM1 to the endoplasmic reticulum-plasma membrane junctions.
Application : WB
Reactivity : Mouse
Submit a Reference

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 639