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PDIR antibody [N1N3]

Anti-PDIR antibody [N1N3] used in Western Blot (WB). GTX115511
Anti-PDIR antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115511
Anti-PDIR antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX115511
Anti-PDIR antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX115511

Cat. No. GTX115511

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all PDIR products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Positive Control

HepG2

Predict Reactivity

Rat, Sheep, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.52 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PDIR. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619349

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein disulfide isomerase family A member 5 , PDIR

Cellular Localization

Endoplasmic reticulum lumen

Database

Research Area

DATA IMAGES

Anti-PDIR antibody [N1N3] used in Western Blot (WB). GTX115511

GTX115511 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
12% SDS PAGE
GTX115511 diluted at 1:1000

Anti-PDIR antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115511

GTX115511 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A549, using PDIR(GTX115511) antibody at 1:200 dilution.

Anti-PDIR antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX115511

GTX115511 IHC-P Image

PDIR antibody [N1N3] detects PDIR protein at cytoplasm in mouse pancreas by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
PDIR antibody [N1N3] (GTX115511) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PDIR antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX115511

GTX115511 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using PDIR(GTX115511) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169