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Mouse Glyoxalase I protein, His tag (active)

Mouse Glyoxalase I protein, His tag (active). GTX67015-pro

Cat. No. GTX67015-pro

Application

Functional Assay

Species

Mouse
Package
10 μg ($219)

APPLICATION

Application Note

Specific activity is > 210 units/mg, and is defined as the amount of enzyme that will form 1.0 μmole of S-lactoylglutathione from methylglyoxal and reduced glutathione per minute at pH 6.5 at 25ºC.

Observed MW

28-40 kDa.

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Region/Sequence

Full length protein, C-terminal His-Tag; Length: 192 a.a. Sequence: MAEPQPASSG LTDETAFSCC SDPDPSTKDF LLQQTMLRIK DPKKSLDFYT RVLGLTLLQK LDFPAMKFSL YFLAYEDKND IPKDKSEKTA WTFSRKATLE LTHNWGTEDD ETQSYHNGNS DPRGFGHIGI AVPDVYSACK RFEELGVKFV KKPDDGKMKG LAFIQDPDGY WIEILNPNKI ATIILEHHHH HH

Expression System

Baculovirus

Purity

> 95% by SDS-PAGE.

Endotoxin

< 1.0 EU/μg (determined by LAL method)

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

glyoxalase 1 , 0610009E22Rik , 1110008E19Rik , 2510049H23Rik , AW550643 , GLY1 , Glo-1 , Glo-1r , Glo-1s , Glo1-r , Glo1-s , Qglo

Database

Research Area

DATA IMAGES

Mouse Glyoxalase I protein, His tag (active). GTX67015-pro

GTX67015-pro Image

3 μg of GTX67015-pro Mouse Glyoxalase I protein (active) by SDS-PAGE under reducing condition and visualized by coomassie blue stain

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 219