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Lipoic acid synthetase antibody [N1C2]

Anti-Lipoic acid synthetase antibody [N1C2] used in Western Blot (WB). GTX115528

Cat. No. GTX115528

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Lipoic acid synthetase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

Raji

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Lipoic acid synthetase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10624413

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

lipoic acid synthetase , HGCLAS , HUSSY-01 , LAS , LIP1 , LS , PDHLD

Cellular Localization

Mitochondrion

Background

The protein encoded by this gene belongs to the biotin and lipoic acid synthetases family. It localizes in mitochondrion and plays an important role in alpha-(+)-lipoic acid synthesis. It may also function in the sulfur insertion chemistry in lipoate biosynthesis. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Lipoic acid synthetase antibody [N1C2] used in Western Blot (WB). GTX115528

GTX115528 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
10% SDS PAGE
GTX115528 diluted at 1:2000

REFERENCE

Application Reference

Xu L et al. PLoS One 2016; 11 (10) : e0163208 Influence of Different Levels of Lipoic Acid Synthase Gene Expression on Diabetic Nephropathy.
Application : WB
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169