APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:10000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2 , Rat2
Predict Reactivity
Bovine(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Tara. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10624951
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
TRIO and F-actin binding protein , DFNB28 , HRIHFB2122 , TAP68 , TARA , dJ37E16.4
Cellular Localization
Nucleus , Cytoplasm , cytoskeleton
Background
This gene encodes a protein with an N-terminal pleckstrin homology domain and a C-terminal coiled-coil region. The protein interacts with trio, which is involved with neural tissue development and controlling actin cytoskeleton organization, cell motility and cell growth. The protein also associates with F-actin and stabilizes F-actin structures. Mutations in this gene have been associated with a form of autosomal recessive nonsyndromic deafness. Multiple alternatively spliced transcript variants that would encode different isoforms have been found for this gene, however some transcripts may be subject to nonsense-mediated decay (NMD). [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-Tara antibody [C2C3-2], C-term used in Western Blot (WB). GTX110158](/templates/default/js/lightbox/images/loading.gif "Anti-Tara antibody [C2C3-2], C-term used in Western Blot (WB). GTX110158")
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GTX110158 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tara antibody [C2C3-2], C-term (GTX110158) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX110158 IHC-P Image
Tara antibody [C2C3-2], C-term detects Tara protein at cytoplasm in human lung cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
Tara antibody [C2C3-2], C-term (GTX110158) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX110158 WB Image
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Tara antibody [C2C3-2], C-term (GTX110158) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX110158 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Tara(GTX110158) antibody at 1:200 dilution.
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REFERENCE
There are currently no references for Tara antibody [C2C3-2], C-term (GTX110158). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Tara antibody [C2C3-2], C-term (GTX110158). Be the first to share your experience with this product.
![Anti-Tara antibody [C2C3-2], C-term used in Western Blot (WB). GTX110158](/upload/website/prouct_img/small/GTX110158/GTX110158_40205_20160317_WB_w_23060500_421.webp "Anti-Tara antibody [C2C3-2], C-term used in Western Blot (WB). GTX110158")
![Anti-Tara antibody [C2C3-2], C-term used in IHC (Paraffin sections) (IHC-P). GTX110158](/upload/website/prouct_img/small/GTX110158/GTX110158_40205_20150529_IHC_w_23060500_357.webp "Anti-Tara antibody [C2C3-2], C-term used in IHC (Paraffin sections) (IHC-P). GTX110158")
![Anti-Tara antibody [C2C3-2], C-term used in Western Blot (WB). GTX110158](/upload/website/prouct_img/small/GTX110158/GTX110158_40205_20160324_WB_R_w_23060500_730.webp "Anti-Tara antibody [C2C3-2], C-term used in Western Blot (WB). GTX110158")
![Anti-Tara antibody [C2C3-2], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110158](/upload/website/prouct_img/small/GTX110158/GTX110158_40205_IFA_w_23060500_324.webp "Anti-Tara antibody [C2C3-2], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110158")
![Anti-Tara antibody [C2C3-2], C-term used in Western Blot (WB). GTX110158](/upload/website/prouct_img/normal/GTX110158/GTX110158_40205_20160317_WB_w_23060500_421.webp "Anti-Tara antibody [C2C3-2], C-term used in Western Blot (WB). GTX110158")
![Anti-Tara antibody [C2C3-2], C-term used in IHC (Paraffin sections) (IHC-P). GTX110158](/upload/website/prouct_img/normal/GTX110158/GTX110158_40205_20150529_IHC_w_23060500_357.webp "Anti-Tara antibody [C2C3-2], C-term used in IHC (Paraffin sections) (IHC-P). GTX110158")
![Anti-Tara antibody [C2C3-2], C-term used in Western Blot (WB). GTX110158](/upload/website/prouct_img/normal/GTX110158/GTX110158_40205_20160324_WB_R_w_23060500_730.webp "Anti-Tara antibody [C2C3-2], C-term used in Western Blot (WB). GTX110158")
![Anti-Tara antibody [C2C3-2], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110158](/upload/website/prouct_img/normal/GTX110158/GTX110158_40205_IFA_w_23060500_324.webp "Anti-Tara antibody [C2C3-2], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110158")