Form
Liquid
Buffer
20mM Tris-HCl buffer (pH7.5), 0.2M NaCl, 30% Glycerol, 1mM DTT
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.25 mg/ml (Please refer to the vial label for the specific concentration.)
Region/Sequence
Full length protein, N-terminal His-Tag; MGSSHHHHHH SSGLVPRGSH MGSMGKDYYC ILGIEKGASD EDIKKAYRKQ ALKFHPDKNK SPQAEEKFKE VAEAYEVLSD PKKREIYDQF GEEGLKGGAG GTDGQGGTFR YTFHGDPHAT FAAFFGGSNP FEIFFGRRMG GGRDSEEMEI DGDPFSAFGF SMNGYPRDRN SVGPSRLKQD PPVIHELRVS LEEIYSGCTK RMKISRKRLN ADGRSYRSED KILTIEIKKG WKEGTKITFP REGDETPNSI PADIVFIIKD KDHPKFKRDG SNIIYTAKIS LREALCGCSI NVPTLDGRNI PMSVNDIVKP GMRRRIIGYG LPFPKNPDQR GDLLIEFEVS FPDTISSSSK EVLRKHLPAS
Expression System
E. coli
Purity
> 90% by SDS-PAGE.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Synonyms
DnaJ heat shock protein family (Hsp40) member B4 , DNAJW , DjB4 , HLJ1
Cellular Localization
Cytoplasm,Cell membrane
Background
The protein encoded by this gene is a molecular chaperone, tumor suppressor, and member of the heat shock protein-40 family. The encoded protein binds the cell adhesion protein E-cadherin and targets it to the plasma membrane. This protein also binds incorrectly folded E-cadherin and targets it for endoplasmic reticulum-associated degradation. This gene is a strong tumor suppressor for colorectal carcinoma, and downregulation of it may serve as a good biomarker for predicting patient outcomes. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015]
Database
Research Area