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Calpain 11 antibody

Anti-Calpain 11 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115584
Anti-Calpain 11 antibody used in IHC (Paraffin sections) (IHC-P). GTX115584
Anti-Calpain 11 antibody used in Western Blot (WB). GTX115584

Cat. No. GTX115584

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Calpain 11 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

84 kDa. ( Note )

Positive Control

HeLa , NT2D1

Predict Reactivity

Mouse, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Calpain 11. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10620270

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

calpain 11 , calpain11

Cellular Localization

Acrosome

Background

Calpains constitute a family of intracellular calcium-dependent cysteine proteases. There are eight members in this superfamily. They consist of a variable 80 kDa subunit and an invariant 30 kDa subunit. This calpain protein appears to have protease activity and calcium-binding ability. A similar mouse protein may play a functional role in spermatogenesis and in the regulation of calcium-dependent signal transduction events during meiosis. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Calpain 11 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115584

GTX115584 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using Calpain 11(GTX115584) antibody (Green) at 1:200 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-Calpain 11 antibody used in IHC (Paraffin sections) (IHC-P). GTX115584

GTX115584 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SCM1 xenograft, using Calpain 11(GTX115584) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Calpain 11 antibody used in Western Blot (WB). GTX115584

GTX115584 WB Image

Sample (30 ug of whole cell lysate)
A: NT2D1
B: Hela
7.5% SDS PAGE
GTX115584 diluted at 1:5000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169