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Trehalase antibody [N1N3]

Anti-Trehalase antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX109979
Anti-Trehalase antibody [N1N3] used in Western Blot (WB). GTX109979
Anti-Trehalase antibody [N1N3] used in Western Blot (WB). GTX109979

Cat. No. GTX109979

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Trehalase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

67 kDa. ( Note )

Positive Control

Mouse stomach , A431

Predict Reactivity

Rat, Rabbit, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Trehalase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952380

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

trehalase , TRE , TREA , TREHD

Cellular Localization

Cell membrane; Lipid-anchor , GPI-anchor

Background

This gene encodes an enzyme that hydrolyses trehalose, a disaccharide formed from two glucose molecules found mainly in fungi, plants, and insects. A partial duplication of this gene is located adjacent to this locus on chromosome 11. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Trehalase antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX109979

GTX109979 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U87 xenograft , using Trehalase(GTX109979) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Trehalase antibody [N1N3] used in Western Blot (WB). GTX109979

GTX109979 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
7.5% SDS PAGE
GTX109979 diluted at 1:1000

Anti-Trehalase antibody [N1N3] used in Western Blot (WB). GTX109979

GTX109979 WB Image

Sample (50 ug of whole cell lysate)
A: mouse stomach
7.5% SDS PAGE
GTX109979 diluted at 1:3000

REFERENCE

Application Reference

Muncan V et al. Nat Commun 2011; 2 : 452 Blimp1 regulates the transition of neonatal to adult intestinal epithelium.
Application : IHC-P
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169