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USP18 antibody

Anti-USP18 antibody used in IHC (Paraffin sections) (IHC-P). GTX04331
Anti-USP18 antibody used in Western Blot (WB). GTX04331
Anti-USP18 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04331
Anti-USP18 antibody used in IHC (Paraffin sections) (IHC-P). GTX04331
Anti-USP18 antibody used in IHC (Paraffin sections) (IHC-P). GTX04331
Anti-USP18 antibody used in IHC (Paraffin sections) (IHC-P). GTX04331

Cat. No. GTX04331

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all USP18 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000 - 1:5000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.3, 50% Glycerol

Preservative

0.05% Proclin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 200-300 of human USP18 (NP_059110.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ubiquitin specific peptidase 18 , ISG43 , PTORCH2 , UBP43

Cellular Localization

Cytoplasm , Nucleus

Background

The protein encoded by this gene belongs to the ubiquitin-specific proteases (UBP) family of enzymes that cleave ubiquitin from ubiquitinated protein substrates. It is highly expressed in liver and thymus, and is localized to the nucleus. This protein efficiently cleaves only ISG15 (a ubiquitin-like protein) fusions, and deletion of this gene in mice results in a massive increase of ISG15 conjugates in tissues, indicating that this protein is a major ISG15-specific protease. Mice lacking this gene are also hypersensitive to interferon, suggesting a function of this protein in downregulating interferon responses, independent of its isopeptidase activity towards ISG15. [provided by RefSeq, Sep 2011]

Database

Research Area

DATA IMAGES

Anti-USP18 antibody used in IHC (Paraffin sections) (IHC-P). GTX04331

GTX04331 IHC-P Image

IHC-P analysis of human colon carcinoma using GTX04331 USP18 antibody.
Antigen retrieval : Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0.
Dilution : 1:20

Anti-USP18 antibody used in Western Blot (WB). GTX04331

GTX04331 WB Image

WB analysis of various cell lysates using GTX04331 USP18 antibody.
Lane 1 : HepG2 whole cell lysate
Lane 2 : mouse liver tissue lysate
Dilution : 1:2000
Loading : 25μg

Anti-USP18 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04331

GTX04331 ICC/IF Image

ICC/IF analysis of MCF7 cells using GTX04331 USP18 antibody.
Orange : Primary antibody
Blue : DAPI
Dilution : 1:200

Anti-USP18 antibody used in IHC (Paraffin sections) (IHC-P). GTX04331

GTX04331 IHC-P Image

IHC-P analysis of human colon tissue using GTX04331 USP18 antibody.
Antigen retrieval : Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0.
Dilution : 1:20

Anti-USP18 antibody used in IHC (Paraffin sections) (IHC-P). GTX04331

GTX04331 IHC-P Image

IHC-P analysis of rat brain tissue using GTX04331 USP18 antibody.
Antigen retrieval : Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0.
Dilution : 1:20

Anti-USP18 antibody used in IHC (Paraffin sections) (IHC-P). GTX04331

GTX04331 IHC-P Image

IHC-P analysis of mouse spinal cord using GTX04331 USP18 antibody.
Antigen retrieval : Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0.
Dilution : 1:20

REFERENCE

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REVIEW

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Package List Price ($)
$ 399