GeneTex

  • Country / Location Selection

United States (US)

KLF8 antibody

Anti-KLF8 antibody used in Western Blot (WB). GTX22747
Anti-KLF8 antibody used in IHC (Paraffin sections) (IHC-P). GTX22747

Cat. No. GTX22747

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
50 μg ($399)
See all KLF8 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-3μg/ml
IHC-P 3.75μg/ml
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX22747-PEP).

Product Note

This antibody is expected to recognise isoform 1 (NP_009181.2) and isoform 2 (NP_001152768.1).

Predict Reactivity

Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence VDMDKLINNLEVQ-C, from the N Terminus of the protein sequence according to NP_009181.2; NP_001152768.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Kruppel like factor 8 , BKLF3 , ZNF741

Cellular Localization

Nucleus

Background

This gene encodes a protein which is a member of the Sp/KLF family of transcription factors. Members of this family contain a C-terminal DNA-binding domain with three Kruppel-like zinc fingers. The encoded protein is thought to play an important role in the regulation of epithelial to mesenchymal transition, a process which occurs normally during development but also during metastasis. A pseudogene has been identified on chromosome 16. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009]

Database

Research Area

DATA IMAGES

Anti-KLF8 antibody used in Western Blot (WB). GTX22747

GTX22747 WB Image

WB analysis of human kidney lysate using GTX22747 KLF8 antibody.
Dilution : 2μg/ml
Loading : 30μg protein in RIPA buffer

Anti-KLF8 antibody used in IHC (Paraffin sections) (IHC-P). GTX22747

GTX22747 IHC-P Image

IHC-P analysis of human small intestine using GTX22747 KLF8 antibody.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75μg/ml

REFERENCE

There are currently no references for KLF8 antibody (GTX22747). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for KLF8 antibody (GTX22747). Be the first to share your experience with this product.
Submit a Review
SDS
Sodium Azide.pdf
Package List Price ($)
$ 399