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MGAT4A antibody [C1C3]

Anti-MGAT4A antibody [C1C3] used in Western Blot (WB). GTX119640
Anti-MGAT4A antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119640
Anti-MGAT4A antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX119640

Cat. No. GTX119640

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all MGAT4A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

62 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human MGAT4A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617950

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A , GNT-IV , GNT-IVA , GnT-4a

Cellular Localization

Alpha-1 , 3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A: Golgi apparatus membrane; Single-pass type II membrane protein , 3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A soluble form: Secreted

Background

This gene encodes a key glycosyltransferase that regulates the formation of tri- and multiantennary branching structures in the Golgi apparatus. The encoded protein, in addition to the related isoenzyme B, catalyzes the transfer of N-acetylglucosamine (GlcNAc) from UDP-GlcNAc in a beta-1,4 linkage to the Man-alpha-1,3-Man-beta-1,4-GlcNAc arm of R-Man-alpha-1,6(GlcNAc-beta-1,2-Man-alpha-1,3)Man-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1-Asn. The encoded protein may play a role in regulating the availability of serum glycoproteins, oncogenesis, and differentiation. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MGAT4A antibody [C1C3] used in Western Blot (WB). GTX119640

GTX119640 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
7.5% SDS PAGE
GTX119640 diluted at 1:1000

Anti-MGAT4A antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119640

GTX119640 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using MGAT4A(GTX119640) antibody at 1:200 dilution.

Anti-MGAT4A antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX119640

GTX119640 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H358 xenograft, using MGAT4A(GTX119640) antibody at 1:250 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169