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Cytoglobin antibody

Anti-Cytoglobin antibody used in Western Blot (WB). GTX117571
Anti-Cytoglobin antibody used in Western Blot (WB). GTX117571
Anti-Cytoglobin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117571
Anti-Cytoglobin antibody used in Western Blot (WB). GTX117571

Cat. No. GTX117571

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Cytoglobin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

IMR32 , Mouse heart , PC-12

Predict Reactivity

Sheep, Bovine, Dog, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cytoglobin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11168273

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cytoglobin , HGB , STAP

Cellular Localization

Cytoplasm

Background

Cytoglobin is a ubiquitously expressed hexacoordinate hemoglobin that may facilitate diffusion of oxygen through tissues, scavenge nitric oxide or other reactive oxygen species, or serve a protective function during oxidative stress (Trent and Hargrove, 2002 [PubMed 11893755]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-Cytoglobin antibody used in Western Blot (WB). GTX117571

GTX117571 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Cytoglobin antibody (GTX117571) diluted at 1:1000.

Anti-Cytoglobin antibody used in Western Blot (WB). GTX117571

GTX117571 WB Image

Sample (30 ug of whole cell lysate)
A: IMR32
12% SDS PAGE
GTX117571 diluted at 1:2000

Anti-Cytoglobin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117571

GTX117571 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using Cytoglobin(GTX117571) antibody at 1:500 dilution.

Anti-Cytoglobin antibody used in Western Blot (WB). GTX117571

GTX117571 WB Image

Sample (50 ug of whole cell lysate)
A: mouse heart
12% SDS PAGE
GTX117571 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169