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HAUS1 antibody [N1C3]

Anti-HAUS1 antibody [N1C3] used in Western Blot (WB). GTX118733
Anti-HAUS1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118733

Cat. No. GTX118733

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all HAUS1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Predict Reactivity

Mouse, Rat, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.43 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human HAUS1 Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617266

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

HAUS augmin like complex subunit 1 , CCDC5 , HEI-C , HEIC , HsT1461

Cellular Localization

Cytoplasm , Centrosome , Spindle

Background

HAUS1 is 1 of 8 subunits of the 390-kD human augmin complex, or HAUS complex. The augmin complex was first identified in Drosophila, and its name comes from the Latin verb 'augmentare,' meaning 'to increase.' The augmin complex is a microtubule-binding complex involved in microtubule generation within the mitotic spindle and is vital to mitotic spindle assembly (Goshima et al., 2008 [PubMed 18443220]; Uehara et al., 2009 [PubMed 19369198]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-HAUS1 antibody [N1C3] used in Western Blot (WB). GTX118733

GTX118733 WB Image

IP-WB assay to show that Hice1 (GTX113617) co-immunoprecipitated with other Augmin components HAUS6 (GTX118732), HAUS2 (GTX118734) and HAUS1 (GTX118733) in U2OS cells.

Anti-HAUS1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118733

GTX118733 ICC/IF Image

HAUS1(CCDC5 ) antibody [N1C3] detects HAUS1(CCDC5 ) protein at centrosome by immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min.
Green: HAUS1(CCDC5 ) protein stained by HAUS1(CCDC5 ) antibody [N1C3] (GTX118733) diluted at 1:1000.
Blue: Hoechst 33342 staining.

REFERENCE

Application Reference

Tsai CY et al. J Biol Chem 2011; 286 (34) : 30097-106 Aurora-A phosphorylates Augmin complex component Hice1 protein at an N-terminal serine/threonine cluster to modulate its microtubule binding activity during spindle assembly.
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169