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CIS antibody [C2C3], C-term

Anti-CIS antibody [C2C3], C-term used in Western Blot (WB). GTX100216
Anti-CIS antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100216
Anti-CIS antibody [C2C3], C-term used in Western Blot (WB). GTX100216
Anti-CIS antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100216

Cat. No. GTX100216

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all CIS products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

HeLa , PC-12 , Rat2

Predict Reactivity

Mouse, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human CIS. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240630

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cytokine inducible SH2 containing protein , BACTS2 , CIS , CIS-1 , G18 , SOCS

Background

The protein encoded by this gene contains a SH2 domain and a SOCS box domain. The protein thus belongs to the cytokine-induced STAT inhibitor (CIS), also known as suppressor of cytokine signaling (SOCS) or STAT-induced STAT inhibitor (SSI), protein family. CIS family members are known to be cytokine-inducible negative regulators of cytokine signaling. The expression of this gene can be induced by IL2, IL3, GM-CSF and EPO in hematopoietic cells. Proteasome-mediated degradation of this protein has been shown to be involved in the inactivation of the erythropoietin receptor. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CIS antibody [C2C3], C-term used in Western Blot (WB). GTX100216

GTX100216 WB Image

Sample (1 ug of whole cell lysate)
A: HeLa
10% SDS PAGE
GTX100216 diluted at 1:10000

Anti-CIS antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100216

GTX100216 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using CIS(GTX100216) antibody at 1:200 dilution.

Anti-CIS antibody [C2C3], C-term used in Western Blot (WB). GTX100216

GTX100216 WB Image

CIS antibody [C2C3], C-term detects CISH protein by Western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat2 whole cell lysate/extract
12 % SDS-PAGE
CIS antibody [C2C3], C-term (GTX100216) dilution: 1:1000

Anti-CIS antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100216

GTX100216 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human lung cancer, using CIS(GTX100216) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Peng HY et al. Neoplasia 2020; 22 (11) : 554-565 MiR-944/CISH mediated inflammation via STAT3 is involved in oral cancer malignance by cigarette smoking.
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 399
$ 169