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FE65 antibody

KO/KD
Anti-FE65 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25668
Anti-FE65 antibody used in Western Blot (WB). GTX25668
Anti-FE65 antibody used in Western Blot (WB). GTX25668

Cat. No. GTX25668

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, FACS

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all FE65 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-2 μg/ml
ICC/IF 2 μg/ml
FACS 3-5 μg/10⁶ cells
Not tested in other applications.

Calculated MW

77 kDa. ( Note )

Product Note

This antibody does not detect endogenous levels of Fe65.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Synthetic Peptide: P(352) Q E E E K L S Q R N A N P G I K C(369)

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

amyloid beta (A4) precursor protein-binding, family B, member 1 , Fe65 , Rir

Cellular Localization

Cell membrane,Cytoplasm,Nucleus,Nucleus speckle

Background

The major transcript of the Fe65 gene is a neuron-specific mRNA that encodes a nuclear protein whose amino-terminal domain strongly activates the transcription of reporter genes. Fe65 has been implicated in a regulatory and cell signaling mechanism because it contains two different motifs involved in protein binding; a WW domain (a variant of Src homology 3 domains) and a phosphotyrosine interaction domain (PID). Interestingly, the PID domain binds to the same motif present in the conserved cytoplasmic domains of the beta PP and beta PP-like proteins. RNA analyses revealed that Fe65 is predominantly expressed in the brain and in the regions most affected by Alzheimer’s disease-associated neuropathology.

Database

Research Area

DATA IMAGES

Anti-FE65 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX25668

GTX25668 ICC/IF Image

ICC/IF analysis of SH-SY5Y cells using GTX25668 FE65 antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 2 μg/ml in 0.1% BSA and incubated for 3 hours at room temperature

Anti-FE65 antibody used in Western Blot (WB). GTX25668

GTX25668 WB Image

KO/KD

WB analysis of pulldown material form mouse brain lysate using GTX25668 FE65 antibody.
Lane 1 : an immunopurified sample from a full-length Fe65 Knockout mouse
Lane 2 : an immunopurified sample from an Fe65 wild type mouse

Anti-FE65 antibody used in Western Blot (WB). GTX25668

GTX25668 WB Image

WB analysis of membrane enriched extracts (30 μg lysate) of U-87 MG (Lane 1), SH-SY5Y (Lane 2), NTERA-2 (Lane 3), U-2 OS (Lane 4), A549 (Lane 5), Mouse Brain (Lane 6) and PC-12 (Lane 7) using GTX25668 FE65 antibody.
Dilution : 1-2 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399