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SEPT12 antibody

Anti-SEPT12 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119571
Anti-SEPT12 antibody used in IHC (Paraffin sections) (IHC-P). GTX119571
Anti-SEPT12 antibody used in Western Blot (WB). GTX119571

Cat. No. GTX119571

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all SEPT12 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Positive Control

Jurkat

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.84 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SEPT12. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617709

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

septin 12 , SPGF10

Cellular Localization

Cytoplasm , Spindle

Background

Septins, such as SEPT12, are conserved GTP-binding proteins that function as dynamic, regulatable scaffolds for the recruitment of other proteins. They are involved in membrane dynamics, vesicle trafficking, apoptosis, and cytoskeleton remodeling, as well as infection, neurodegeneration, and neoplasia (Hall et al., 2005 [PubMed 15915442]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-SEPT12 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119571

GTX119571 ICC/IF Image

SEPT12 antibody detects SEPT12 protein at cytoskeleton by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: SEPT12 protein stained by SEPT12 antibody (GTX119571) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-SEPT12 antibody used in IHC (Paraffin sections) (IHC-P). GTX119571

GTX119571 IHC-P Image

Immunohistochemical analysis of paraffin-embedded hela xenograft, using SEPT12(GTX119571) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SEPT12 antibody used in Western Blot (WB). GTX119571

GTX119571 WB Image

Sample (30 ug of whole cell lysate)
A: Jurkat
10% SDS PAGE
GTX119571 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169