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EGFLAM antibody [C2C3], C-term

Anti-EGFLAM antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX117855
Anti-EGFLAM antibody [C2C3], C-term used in Western Blot (WB). GTX117855
Anti-EGFLAM antibody [C2C3], C-term used in Western Blot (WB). GTX117855

Cat. No. GTX117855

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all EGFLAM products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

111 kDa. ( Note )

Positive Control

Mouse brain , 293T

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human EGFLAM. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618771

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

EGF like, fibronectin type III and laminin G domains , AGRINL , AGRNL , PIKA

Cellular Localization

Secreted , extracellular space , extracellular matrix , Cell junction , synapse

Background

Involved in both the retinal photoreceptor ribbon synapse formation and physiological functions of visual perception. Necessary for proper bipolar dendritic tip apposition to the photoreceptor ribbon synapse. Promotes matrix assembly and cell adhesiveness.

Database

Research Area

DATA IMAGES

Anti-EGFLAM antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX117855

GTX117855 IHC-P Image

EGFLAM antibody [C2C3], C-term detects EGFLAM protein at cytosol on mouse brain stem by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain stem.
EGFLAM antibody [C2C3], C-term (GTX117855) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-EGFLAM antibody [C2C3], C-term used in Western Blot (WB). GTX117855

GTX117855 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
7.5% SDS PAGE
GTX117855 diluted at 1:1000

Anti-EGFLAM antibody [C2C3], C-term used in Western Blot (WB). GTX117855

GTX117855 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
5% SDS PAGE
GTX117855 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169