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DNMT3A antibody [64B814.1]

Overexpression
Anti-DNMT3A antibody [64B814.1] used in Western Blot (WB). GTX13887
Anti-DNMT3A antibody [64B814.1] used in Flow cytometry (FACS). GTX13887
Anti-DNMT3A antibody [64B814.1] used in Western Blot (WB). GTX13887

Cat. No. GTX13887

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 64B814.1

Isotype

 IgG1

Application

 WB, ICC/IF, FACS

Reactivity

 Human, Mouse
Package
100 μg ($429)
See all DNMT3A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 3 μg/ml
ICC/IF 5 μg/ml
FACS Assay dependent
Not tested in other applications.

Calculated MW

102 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

This antibody was raised against bacteria expressed HIS-tag recombinant mouse Dnmt3a.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DNA methyltransferase 3A , MmuIIIA

Cellular Localization

Nucleus,Cytoplasm

Background

Required for genome wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZNF238. Can actively repress transcription through the recruitment of HDAC activity.

Database

Research Area

DATA IMAGES

Anti-DNMT3A antibody [64B814.1] used in Western Blot (WB). GTX13887

GTX13887 WB Image

WB analysis of HEK293 overexpressing mouse DNMT3A (Lanes 1) or mouse DNMT3B (Lane 2) cell lysate using GTX13887 DNMT3A antibody [64B814.1].
Dilution : 2 μg/ml

Anti-DNMT3A antibody [64B814.1] used in Flow cytometry (FACS). GTX13887

GTX13887 FACS Image

FACS (Intracellular staining) analysis of HeLa cells using GTX13887 DNMT3A antibody [64B814.1].
Blue : Primary antibody
Orange : isotype control
Fixation : 4% PFA

Anti-DNMT3A antibody [64B814.1] used in Western Blot (WB). GTX13887

GTX13887 WB Image

Overexpression

WB analysis of HEK293 overexpressing DNMT3A (A) or mock (B) cell lysate using GTX13887 DNMT3A antibody [64B814.1].
Dilution : 1 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 429