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UPRT antibody

Anti-UPRT antibody used in IHC (Paraffin sections) (IHC-P). GTX120370
Anti-UPRT antibody used in Western Blot (WB). GTX120370
Anti-UPRT antibody used in IHC (Paraffin sections) (IHC-P). GTX120370

Cat. No. GTX120370

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all UPRT products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

A549

Predict Reactivity

Zebrafish, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human UPRT. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10732543

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

uracil phosphoribosyltransferase homolog , FUR1 , UPP

Cellular Localization

Cytoplasm , Nucleus

Background

This gene encodes uracil phosphoribosyltransferase, which catalyzes the conversion of uracil and 5-phosphoribosyl-1-R-diphosphate to uridine monophosphate (UMP). This reaction is an important part of nucleotide metabolism, specifically the pyrimidine salvage pathway. The enzyme localizes to the nucleus and cytoplasm. The protein is a potential target for rational design of drugs to treat parasitic infections and cancer. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-UPRT antibody used in IHC (Paraffin sections) (IHC-P). GTX120370

GTX120370 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using UPRT(GTX120370) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-UPRT antibody used in Western Blot (WB). GTX120370

GTX120370 WB Image

Sample (30 ug of whole cell lysate)
A: A549
12% SDS PAGE
GTX120370 diluted at 1:500

Anti-UPRT antibody used in IHC (Paraffin sections) (IHC-P). GTX120370

GTX120370 IHC-P Image

UPRT antibody detects UPRT protein at cytosol and nucleus on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
UPRT antibody (GTX120370) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169