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Cytokeratin 72 antibody

Anti-Cytokeratin 72 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117239
Anti-Cytokeratin 72 antibody used in Western Blot (WB). GTX117239
Anti-Cytokeratin 72 antibody used in IHC (Paraffin sections) (IHC-P). GTX117239

Cat. No. GTX117239

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Cytokeratin 72 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

NT2D1

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cytokeratin 72. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11173698

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

keratin 72 , K6IRS2 , K6irs , KRT6 , KRT6IRS2

Background

Keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells. The type II keratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This gene encodes a type II keratin that is specifically expressed in the inner root sheath of hair follicles. The type II keratins are clustered in a region of chromosome 12q12-q13. Multiple transcript variants encoding different isoforms have been found for this gene.

Database

Research Area

DATA IMAGES

Anti-Cytokeratin 72 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117239

GTX117239 ICC/IF Image

Cytokeratin 72 antibody detects KRT72 protein at cytoplasm by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.
Green: KRT72 protein stained by Cytokeratin 72 antibody (GTX117239) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-Cytokeratin 72 antibody used in Western Blot (WB). GTX117239

GTX117239 WB Image

Sample (30 ug of whole cell lysate)
A: NT2D1
10% SDS PAGE
GTX117239 diluted at 1:1000

Anti-Cytokeratin 72 antibody used in IHC (Paraffin sections) (IHC-P). GTX117239

GTX117239 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using KRT72(GTX117239) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169