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ZNF599 antibody

Orthogonal
Anti-ZNF599 antibody used in Western Blot (WB). GTX119526
Anti-ZNF599 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119526
Anti-ZNF599 antibody used in IHC (Paraffin sections) (IHC-P). GTX119526

Cat. No. GTX119526

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all ZNF599 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

67 kDa. ( Note )

Positive Control

SK-N-SH , SK-N-SH nuclear extract

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ZNF599. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885446

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

zinc finger protein 599

Cellular Localization

Nucleus

Background

May be involved in transcriptional regulation.

Database

DATA IMAGES

Anti-ZNF599 antibody used in Western Blot (WB). GTX119526

GTX119526 WB Image

Orthogonal

SK-N-SH whole cell and nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ZNF599 antibody (GTX119526) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ZNF599 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119526

GTX119526 ICC/IF Image

ZNF599 antibody detects ZNF599 protein at nucleus by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ZNF599 protein stained by ZNF599 antibody (GTX119526) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-ZNF599 antibody used in IHC (Paraffin sections) (IHC-P). GTX119526

GTX119526 IHC-P Image

ZNF599 antibody detects ZNF599 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
ZNF599 stained by ZNF599 antibody (GTX119526) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169