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GTX65642-pro Image
The effects on phospho-ERK1/2 and non-phospho-ERK1/2 by GTX65642-pro Progranulin (mouse) protein in neuronal differentiated mouse P19 cells.Undifferentiated mouse P19 were induced to differentiated in 1μM retinoic acid (RA) in α-minimum essential medium (αMEM) containing 10% heat-treated fetal bovine serum on bacterial grade plates for 3~4 days to allow aggregates to form (generation of embryonic bodies). The aggregates were then plated on tissue culture grade plates in the absence of RA for 3~4days. To examine the induction of signal of phospho-ERK1/2 and ERK1/2 , reactions were carried out at 37ºC over 0, 5, 10, 30, 60, 120mins, respectively by adding the recombinant protein (500ng/ml) to the neuronal differentiated mouse P19 embryonal carcinoma cells, which were maintained with serum starvation for 24hrs. Treatment with GTX65642-pro Progranulin (mouse) protein was performed in lanes 1, 2, 3, 4, 5, and 6 over 0, 5, 10, 30, 60, 120mins, respectively.
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