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GLIS1 antibody

Anti-GLIS1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04548
Anti-GLIS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX04548
Anti-GLIS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX04548
Anti-GLIS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX04548
Anti-GLIS1 antibody used in Western Blot (WB). GTX04548
Anti-GLIS1 antibody used in Flow cytometry (FACS). GTX04548
Anti-GLIS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX04548
Anti-GLIS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX04548

Cat. No. GTX04548

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

 Human
Package
100 μg ($469)
See all GLIS1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.25-0.5 μg/mL
ICC/IF 5 μg/ml/ml
IHC-P 2-5 μg/mL
FACS 1-3 μg/1x10⁶ cells
ELISA 0.1-0.5 μg/mL
Not tested in other applications.

Calculated MW

66 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄

Preservative

No preservatives

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

E.coli-derived human GLIS1 recombinant protein (Position: M1-T620).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

GLIS family zinc finger 1

Cellular Localization

Nucleus

Background

GLIS1 is a GLI (MIM 165220)-related Kruppel-like zinc finger protein that functions as an activator and repressor of transcription (Kim et al., 2002 [PubMed 12042312]).[supplied by OMIM, Mar 2008]

Database

Research Area

DATA IMAGES

Anti-GLIS1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04548

GTX04548 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX04548 GLIS1 antibody.
Green : Primary antibody
Blue : DAPI
Antigen retrieval : IHC enzyme antigen retrieval reagent
Dilution : 5 μg/mL

Anti-GLIS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX04548

GTX04548 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX04548 GLIS1 antibody.
Antigen retrieval : Heat mediated EDTA buffer (pH8.0)
Dilution : 2 μg/mL

Anti-GLIS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX04548

GTX04548 IHC-P Image

IHC-P analysis of human lung cancer tissue using GTX04548 GLIS1 antibody.
Antigen retrieval : Heat mediated EDTA buffer (pH8.0)
Dilution : 2 μg/mL

Anti-GLIS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX04548

GTX04548 IHC-P Image

IHC-P analysis of human esophageal squamous carcinoma tissue using GTX04548 GLIS1 antibody.
Antigen retrieval : Heat mediated EDTA buffer (pH8.0)
Dilution : 2 μg/mL

Anti-GLIS1 antibody used in Western Blot (WB). GTX04548

GTX04548 WB Image

WB analysis of various whole cell lysates using GTX04548 GLIS1 antibody.
Lane 1: 293T whole cell lysate
Lane2 : U-87MG whole cell lysate
Lane3 : PC-3 whole cell lysate
Lane4 : HeLa whole cell lysate
Dilution : 0.5 μg/mL
Loading : 30 μg

Anti-GLIS1 antibody used in Flow cytometry (FACS). GTX04548

GTX04548 FACS Image

FACS analysis of 293T cells using GTX04548 GLIS1 antibody.
Blue : Primary antibody
Green : Isotype control
Red : Unlabelled sample
Dilution : 1 μg/1x10⁶ cells

Anti-GLIS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX04548

GTX04548 IHC-P Image

IHC-P analysis of human colorectal adenocarcinoma tissue using GTX04548 GLIS1 antibody.
Antigen retrieval : Heat mediated EDTA buffer (pH8.0)
Dilution : 2 μg/mL

Anti-GLIS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX04548

GTX04548 IHC-P Image

IHC-P analysis of human ovarian cancer tissue using GTX04548 GLIS1 antibody.
Antigen retrieval : Heat mediated EDTA buffer (pH8.0)
Dilution : 2 μg/mL

REFERENCE

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REVIEW

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Package List Price ($)
$ 469