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HscB antibody [N1C3]

Anti-HscB antibody [N1C3] used in Western Blot (WB). GTX116372

Cat. No. GTX116372

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all HscB products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

Positive Control

293T

Predict Reactivity

Mouse, Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HscB. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10616405

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

HscB mitochondrial iron-sulfur cluster cochaperone , DNAJC20 , HSC20 , JAC1

Cellular Localization

Mitochondrion

Background

May act as a co-chaperone in iron-sulfur cluster assembly in mitochondria.

Database

Research Area

DATA IMAGES

Anti-HscB antibody [N1C3] used in Western Blot (WB). GTX116372

GTX116372 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
12% SDS PAGE
GTX116372 diluted at 1:1000

REFERENCE

Application Reference

Ast T et al. Cell 2019; 177 (6) : 1507-1521.e16 Hypoxia Rescues Frataxin Loss by Restoring Iron Sulfur Cluster Biogenesis.
Lee S et al. Free Radic Biol Med 2018; 126 : 133-141 ChIP-seq analysis reveals alteration of H3K4 trimethylation occupancy in cancer-related genes by cold atmospheric plasma.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169