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SGOL1 antibody

Orthogonal
Anti-SGOL1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117103
Anti-SGOL1 antibody used in Western Blot (WB). GTX117103
Anti-SGOL1 antibody used in Western Blot (WB). GTX117103

Cat. No. GTX117103

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all SGOL1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

64 kDa. ( Note )

Positive Control

HepG2

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SGOL1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11167081

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

shugoshin 1 , CAID , NY-BR-85 , SGO , SGOL1

Cellular Localization

Nucleus , Centromere

Database

Research Area

DATA IMAGES

Anti-SGOL1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117103

GTX117103 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using SGOL1(GTX117103) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-SGOL1 antibody used in Western Blot (WB). GTX117103

GTX117103 WB Image

Sample (30 ug of whole cell lysate)
A: HepG2
12% SDS PAGE
GTX117103 diluted at 1:1000

Anti-SGOL1 antibody used in Western Blot (WB). GTX117103

GTX117103 WB Image

Orthogonal

The data was published in the journal Sci Rep in 2016.PMID: 27539729

REFERENCE

Application Reference

Murakami-Tonami Y et al. Sci Rep 2016; 6 : 31615 SGO1 is involved in the DNA damage response in MYCN-amplified neuroblastoma cells.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169