APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
mouse brain , rat brain
Predict Reactivity
Zebrafish, Bovine, Chicken, Xenopus tropicalis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.59 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Mouse
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of mouse Grp75. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2886909
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
heat shock protein 9 , 74kDa , Csa , Grp75 , Hsc74 , Hsp74 , Hsp74a , Hspa9a , Mortalin , Mot-2 , Mot2 , Mthsp70 , Pbp74
Cellular Localization
Mitochondrion
Background
Implicated in the control of cell proliferation and cellular aging. May also act as a chaperone.
Database
Research Area
DATA IMAGES
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GTX133283 IHC-P Image
Grp75 antibody detects Grp75 protein at cytoplasm in rat kidney by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
Grp75 antibody (GTX133283) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX133283 IHC-P Image
Grp75 antibody detects Grp75 protein at cytoplasm in mouse pancreas by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
Grp75 antibody (GTX133283) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX133283 WB Image
Various tissue extracts (10 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp75 antibody (GTX133283) diluted at 1:2000.
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GTX133283 IHC-Fr Image
Grp75 antibody detects Grp75 protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: Grp75 protein stained by Grp75 antibody (GTX133283) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 10 min
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GTX133283 ICC/IF Image
Grp75 antibody detects Grp75 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Grp75 protein stained by Grp75 antibody (GTX133283) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
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GTX133283 WB Image
Drosophila tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp75 antibody (GTX133283) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
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REVIEW
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