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HuC antibody

Anti-HuC antibody used in IHC (Frozen sections) (IHC-Fr). GTX134128
Anti-HuC antibody used in Western Blot (WB). GTX134128
Anti-HuC antibody used in IHC (Frozen sections) (IHC-Fr). GTX134128
Anti-HuC antibody used in IHC (Paraffin sections) (IHC-P). GTX134128
Anti-HuC antibody used in IHC (Paraffin sections) (IHC-P). GTX134128
Anti-HuC antibody used in IHC (Paraffin sections) (IHC-P). GTX134128
Anti-HuC antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134128

Cat. No. GTX134128

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all HuC products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

mouse brain , rat brain

Predict Reactivity

Human(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.26 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of mouse HuC. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887225

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ELAV like RNA binding protein 3 , 2600009P04Rik , Huc , PLE21 , mHuC

Background

Binds to AU-rich sequences (AREs) of target mRNAs, including VEGF mRNA. May also bind poly-A tracts via RRM 3. May be involved in neuronal differentiation and maintenance.

Database

Research Area

DATA IMAGES

Anti-HuC antibody used in IHC (Frozen sections) (IHC-Fr). GTX134128

GTX134128 IHC-Fr Image

HuC antibody detects HuC protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse hippocampus.
Green: HuC stained by HuC antibody (GTX134128) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-HuC antibody used in Western Blot (WB). GTX134128

GTX134128 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HuC antibody (GTX134128) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HuC antibody used in IHC (Frozen sections) (IHC-Fr). GTX134128

GTX134128 IHC-Fr Image

HuC antibody detects HuC protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse hippocampus.
Green: HuC stained by HuC antibody (GTX134128) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-HuC antibody used in IHC (Paraffin sections) (IHC-P). GTX134128

GTX134128 IHC-P Image

HuC antibody detects HuC protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
HuC stained by HuC antibody (GTX134128) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

Anti-HuC antibody used in IHC (Paraffin sections) (IHC-P). GTX134128

GTX134128 IHC-P Image

HuC antibody detects HuC protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
HuC stained by HuC antibody (GTX134128) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

Anti-HuC antibody used in IHC (Paraffin sections) (IHC-P). GTX134128

GTX134128 IHC-P Image

HuC antibody detects HuC protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
HuC stained by HuC antibody (GTX134128) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

Anti-HuC antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134128

GTX134128 ICC/IF Image

HuC antibody detects HuC protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HuC stained by HuC antibody (GTX134128) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169