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Bone Sialoprotein antibody

Anti-Bone Sialoprotein antibody used in Western Blot (WB). GTX12155
Anti-Bone Sialoprotein antibody used in IHC (Paraffin sections) (IHC-P). GTX12155
Anti-Bone Sialoprotein antibody used in IHC (Paraffin sections) (IHC-P). GTX12155

Cat. No. GTX12155

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Mouse, Rat
Package
100 μg ($449)
See all Bone Sialoprotein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1-0.5μg/ml
IHC-P 0.5-1μg/ml
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.1% Na₂HPO₄, 0.45% NaCl, 2.5% BSA

Preservative

0.025% Thimerosal, 0.025% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

500 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of mouse Bone Sialoprotein(305-324aa SYYKGHGYEGYEGQNYYYHQ), different from the related rat sequence by one amino acid.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_2885566

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

integrin binding sialoprotein , BSP , BSP II , BSPII , Bsp2

Cellular Localization

Secreted

Database

Research Area

DATA IMAGES

Anti-Bone Sialoprotein antibody used in Western Blot (WB). GTX12155

GTX12155 WB Image

WB analysis of various samples using GTX12155 Bone Sialoprotein antibody.
Lane 1 : rat liver tissue lysate
Lane 2 : rat brain tissue lysate
Lane 3 : rat kidney tissue lysate

Anti-Bone Sialoprotein antibody used in IHC (Paraffin sections) (IHC-P). GTX12155

GTX12155 IHC-P Image

IHC-P analysis of rat liver tissue using GTX12155 Bone Sialoprotein antibody.

Anti-Bone Sialoprotein antibody used in IHC (Paraffin sections) (IHC-P). GTX12155

GTX12155 IHC-P Image

IHC-P analysis of mouse pancreas tissue using GTX12155 Bone Sialoprotein antibody.
Antigen retireval : Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins
Dilution : 1μg/ml

REFERENCE

Application Reference

Xue J et al. Commun Biol 2023; 6 (1) : 1120 LncRNA SNHG14 activates autophagy via regulating miR-493-5p/Mef2c axis to alleviate osteoporosis progression.
Jee HK et al. Biomimetics (Basel) 2023; 8 (1) Long-Term Changes in Adipose Tissue in the Newly Formed Bone Induced by Recombinant Human BMP-2 In Vivo.
Hyun Seok et al. Int J Mol Sci 2021; 22 (20) Comparison of Bone Regeneration in Different Forms of Bovine Bone Scaffolds with Recombinant Human Bone Morphogenetic Protein-2.
Application : IHC-P
Reactivity : Rat
Jang E et al. Materials (Basel) 2019; 12 (10) Evaluation of the Bone Regeneration Effect of Recombinant Human Bone Morphogenic Protein-2 on Subperiosteal Bone Graft in the Rat Calvarial Model.
Application : IHC-P
Reactivity : Rat
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SDS
Sodium Azide.pdf
Thimerosal.pdf
Package List Price ($)
$ 449