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ID1 antibody

Comparison Overexpression
Anti-ID1 antibody used in Western Blot (WB). GTX133738
Anti-ID1 antibody used in Western Blot (WB). GTX133738
Anti-ID1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133738
Anti-ID1 antibody used in Western Blot (WB). GTX133738
Anti-ID1 antibody used in Western Blot (WB). GTX133738

Cat. No. GTX133738

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all ID1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Positive Control

ID1-transfected 293T , A431 , HeLa , HepG2 , mouse ESC

Predict Reactivity

Rat, Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.83 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant protein encompassing a sequence within the center region of mouse ID1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887087

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

inhibitor of DNA binding 1 , AI323524 , D2Wsu140e , Idb1 , bHLHb24

Database

Research Area

DATA IMAGES

Anti-ID1 antibody used in Western Blot (WB). GTX133738

GTX133738 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membranes were blotted with ID1 antibody (GTX133738) diluted at 1:500 and competitor's antibody (sc-488) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-ID1 antibody used in Western Blot (WB). GTX133738

GTX133738 WB Image

Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with ID1 antibody (GTX133738) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ID1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133738

GTX133738 ICC/IF Image

ID1 antibody detects ID1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ID1 stained by ID1 antibody (GTX133738) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
Scale bar= 10μm.

Anti-ID1 antibody used in Western Blot (WB). GTX133738

GTX133738 WB Image

Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with ID1 antibody (GTX133738) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ID1 antibody used in Western Blot (WB). GTX133738

GTX133738 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with ID1 antibody (GTX133738) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169