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DDT antibody [N1C3]

Anti-DDT antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX101791
Anti-DDT antibody [N1C3] used in Western Blot (WB). GTX101791
Anti-DDT antibody [N1C3] used in Western Blot (WB). GTX101791
Anti-DDT antibody [N1C3] used in Western Blot (WB). GTX101791

Cat. No. GTX101791

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all DDT products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

13 kDa. ( Note )

Positive Control

Raji , mouse liver , rat liver

Predict Reactivity

Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DDT. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036747

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

D-dopachrome tautomerase , D-DT , DDCT , MIF-2 , MIF2

Cellular Localization

Cytoplasm

Background

D-dopachrome tautomerase converts D-dopachrome into 5,6-dihydroxyindole. The DDT gene is related to the migration inhibitory factor (MIF) in terms of sequence, enzyme activity, and gene structure. DDT and MIF are closely linked on chromosome 22. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-DDT antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX101791

GTX101791 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using DDT(GTX101791) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-DDT antibody [N1C3] used in Western Blot (WB). GTX101791

GTX101791 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
15% SDS PAGE
GTX101791 diluted at 1:500

Anti-DDT antibody [N1C3] used in Western Blot (WB). GTX101791

GTX101791 WB Image

Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with DDT antibody [N1C3] (GTX101791) diluted at 1:500.

Anti-DDT antibody [N1C3] used in Western Blot (WB). GTX101791

GTX101791 WB Image

Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with DDT antibody [N1C3] (GTX101791) diluted at 1:500.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169