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SAP102 antibody [C1C3]

Anti-SAP102 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110289
Anti-SAP102 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX110289
Anti-SAP102 antibody [C1C3] used in Western Blot (WB). GTX110289
Anti-SAP102 antibody [C1C3] used in Western Blot (WB). GTX110289
Anti-SAP102 antibody [C1C3] used in IHC (Frozen sections) (IHC-Fr). GTX110289
Anti-SAP102 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110289
Anti-SAP102 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110289
Anti-SAP102 antibody [C1C3] used in Western Blot (WB). GTX110289
Anti-SAP102 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX110289

Cat. No. GTX110289

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat, Pig
Package
100 μl ($399),
25 μl ($169)
See all SAP102 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

90 kDa. ( Note )

Positive Control

Molt-4 , mouse brain , rat brain

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human SAP102. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950120

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

discs large MAGUK scaffold protein 3 , MRX , MRX90 , NEDLG , PPP1R82 , SAP102 , XLMR

Background

This gene encodes a member of the membrane-associated guanylate kinase protein family. The encoded protein may play a role in clustering of NMDA receptors at excitatory synapses. It may also negatively regulate cell proliferation through interaction with the C-terminal region of the adenomatosis polyposis coli tumor suppressor protein. Mutations in this gene have been associated with X-linked mental retardation. Alternatively spliced transcript variants have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SAP102 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110289

GTX110289 ICC/IF Image

SAP102 antibody [C1C3] detects SAP102 protein at cell body and synaptic vesicles by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SAP102 protein stained by SAP102 antibody [C1C3] (GTX110289) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-SAP102 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX110289

GTX110289 IHC-P Image

SAP102 antibody [C1C3] detects SAP102 protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
SAP102 antibody [C1C3] (GTX110289) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SAP102 antibody [C1C3] used in Western Blot (WB). GTX110289

GTX110289 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SAP102 antibody [C1C3] (GTX110289) diluted at 1:1000.

Anti-SAP102 antibody [C1C3] used in Western Blot (WB). GTX110289

GTX110289 WB Image

Sample (30 ug of whole cell lysate)
A: Molt-4 (GTX27912)
7.5% SDS PAGE
GTX110298 diluted at 1:1000

Anti-SAP102 antibody [C1C3] used in IHC (Frozen sections) (IHC-Fr). GTX110289

GTX110289 IHC-Fr Image

SAP102 antibody [C1C3] detects SAP102 protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: SAP102 antibody [C1C3] stained by SAP102 antibody (GTX110289) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-SAP102 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110289

GTX110289 ICC/IF Image

SAP102 antibody [C1C3] detects SAP102 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SAP102 protein stained by SAP102 antibody [C1C3] (GTX110289) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-SAP102 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110289

GTX110289 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using SAP102(GTX110289) antibody at 1:200 dilution.

Anti-SAP102 antibody [C1C3] used in Western Blot (WB). GTX110289

GTX110289 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX110289 diluted at 1:1000

Anti-SAP102 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX110289

GTX110289 IHC-P Image

SAP102 antibody [C1C3] detects SAP102 protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
SAP102 antibody [C1C3] (GTX110289) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Butko MT et al. Proc Natl Acad Sci U S A 2013; 110 (8) : E726-35 In vivo quantitative proteomics of somatosensory cortical synapses shows which protein levels are modulated by sensory deprivation.
Application : WB
Reactivity : Mouse
Yun-Hong Y et al. Mol Cell Proteomics 2011; 10 (10) : M110.007138 A study of the spatial protein organization of the postsynaptic density isolated from porcine cerebral cortex and cerebellum.
Application : WB
Reactivity : Pig
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169