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DNase I antibody [N1C3]

Anti-DNase I antibody [N1C3] used in Western Blot (WB). GTX109306
Anti-DNase I antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109306
Anti-DNase I antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109306
Anti-DNase I antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109306
Anti-DNase I antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109306

Cat. No. GTX109306

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Monkey
Package
100 μl ($399),
25 μl ($169)
See all DNase I products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Predict Reactivity

Rabbit, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.19 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein corresponding to human DNase I.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950133

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

deoxyribonuclease 1 , DNL1 , DRNI

Cellular Localization

Secreted , Nucleus envelope

Background

This gene encodes a member of the DNase family. This protein is stored in the zymogen granules of the nuclear envelope and functions by cleaving DNA in an endonucleolytic manner. At least six autosomal codominant alleles have been characterized, DNASE1*1 through DNASE1*6, and the sequence of DNASE1*2 represented in this record. Mutations in this gene have been associated with systemic lupus erythematosus (SLE), an autoimmune disease. A recombinant form of this protein is used to treat the one of the symptoms of cystic fibrosis by hydrolyzing the extracellular DNA in sputum and reducing its viscosity. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-DNase I antibody [N1C3] used in Western Blot (WB). GTX109306

GTX109306 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with DNase I antibody [N1C3] (GTX109306) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DNase I antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109306

GTX109306 IHC-P Image

DNase I antibody [N1C3] detects DNase I protein at cytoplasm in human endometrial carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
DNase I antibody [N1C3] (GTX109306) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-DNase I antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109306

GTX109306 ICC/IF Image

DNase I antibody [N1C3] detects DNase I protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: DNase I protein stained by DNase I antibody [N1C3] (GTX109306) diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-DNase I antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109306

GTX109306 IHC-P Image

DNase I antibody [N1C3] detects DNase I protein at cytoplasm in human cervical carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
DNase I antibody [N1C3] (GTX109306) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-DNase I antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109306

GTX109306 IHC-P Image

DNase I antibody [N1C3] detects DNase I protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
DNase I stained by DNase I antibody [N1C3] (GTX109306) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Ueki M et al. Sci Rep 2019; 9 (1) : 13660 Evaluation of the functional effects of genetic variants?issense and nonsense SNPs, indels and copy number variations?n the gene encoding human deoxyribonuclease I potentially implicated in autoimmunity.
Application : WB
Reactivity : Monkey
Lam C et al. Biotechnol Prog 2017; (Epub) Taming hyperactive hDNase I: Stable inducible expression of a hyperactive salt- and actin-resistant variant of human deoxyribonuclease I in CHO cells.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169