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DNMT3B antibody

KO/KD Orthogonal Overexpression
Anti-DNMT3B antibody used in Western Blot (WB). GTX129127
Anti-DNMT3B antibody used in Western Blot (WB). GTX129127
Anti-DNMT3B antibody used in Western Blot (WB). GTX129127
Anti-DNMT3B antibody used in IHC (Paraffin sections) (IHC-P). GTX129127
Anti-DNMT3B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129127
Anti-DNMT3B antibody used in Immunoprecipitation (IP). GTX129127
Anti-DNMT3B antibody used in Western Blot (WB). GTX129127
Anti-DNMT3B antibody used in Western Blot (WB). GTX129127

Cat. No. GTX129127

Reference 17

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all DNMT3B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

96 kDa. ( Note )

Positive Control

HeLa nuclear , GFP-tagged DNMT3B-transfested 293T

Predict Reactivity

Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.29 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human DNMT3B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885904

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DNA methyltransferase 3 beta , ICF , ICF1 , M.HsaIIIB

Cellular Localization

Nucleus

Background

CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. Six alternatively spliced transcript variants have been described. The full length sequences of variants 4 and 5 have not been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-DNMT3B antibody used in Western Blot (WB). GTX129127

GTX129127 WB Image

KO/KD

Wild-type (WT) and DNMT3B knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DNMT3B antibody (GTX129127) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DNMT3B antibody used in Western Blot (WB). GTX129127

GTX129127 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DNMT3B antibody (GTX129127) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DNMT3B antibody used in Western Blot (WB). GTX129127

GTX129127 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DNMT3B antibody (GTX129127) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-DNMT3B antibody used in IHC (Paraffin sections) (IHC-P). GTX129127

GTX129127 IHC-P Image

DNMT3B antibody detects DNMT3B protein at nucleus on human lung carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung carcinoma.
DNMT3B antibody (GTX129127) diluted at 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-DNMT3B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129127

GTX129127 ICC/IF Image

DNMT3B antibody detects DNMT3B protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DNMT3B protein stained by DNMT3B antibody (GTX129127) diluted at 1:200.
Red: phalloidin, a cytoskeleton marker, diluted at 1:50.
Scale bar = 10 μm.

Anti-DNMT3B antibody used in Immunoprecipitation (IP). GTX129127

GTX129127 IP Image

Immunoprecipitation of DNMT3B protein from K562 nuclear extracts using 5 μg of DNMT3B antibody (GTX129127).
Western blot analysis was performed using DNMT3B antibody (GTX129127) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-DNMT3B antibody used in Western Blot (WB). GTX129127

GTX129127 WB Image

Orthogonal

The data was published in the journal Oncotarget in 2018. PMID: 29416619

Anti-DNMT3B antibody used in Western Blot (WB). GTX129127

GTX129127 WB Image

Orthogonal

The data was published in the journal Oncotarget in 2018. PMID: 29416619

REFERENCE

Application Reference

L Zhang et al. Pharmacogn Mag 2024; RIBE from Multiple Irradiation and the Protective Effects of Astragalus Polysaccharides on Methylation and Cancer-associated Proteins in BMSCs
Wu YH et al. J Ovarian Res 2023; 16 (1) : 124 The downregulation of miR-509-3p expression by collagen type XI alpha 1-regulated hypermethylation facilitates cancer progression and chemoresistance via the DNA methyltransferase 1/Small ubiquitin-like modifier-3 axis in ovarian cancer cells.
H Tu et al. Am J Cancer Res 2023; 13 (6) : 2307-2322 Loss of MEG3 contributes to the enhanced migration and invasion in arsenic-induced carcinogenesis through NQO1/FSCN1 pathway
Wu YH et al. Res Sq 2023; The Downregulation of miR-509-3p Expression by Collagen Type XI Alpha 1-Regulated Hypermethylation Facilitates Cancer Progression and Chemoresistance via the DNA Methyltransferase 1/Small Ubiquitin-like Modifier-3 Axis in Ovarian Cancer Cells.
Xu J et al. Cancers (Basel) 2022; 14 (17) DNMT3A/<i>miR-129-2-5p</i>/Rac1 Is an Effector Pathway for <i>SNHG1</i> to Drive Stem-Cell-like and Invasive Behaviors of Advanced Bladder Cancer Cells.
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REVIEW

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Application Reference
L Zhang et al. Pharmacogn Mag 2024; RIBE from Multiple Irradiation and the Protective Effects of Astragalus Polysaccharides on Methylation and Cancer-associated Proteins in BMSCs
Wu YH et al. J Ovarian Res 2023; 16 (1):124 The downregulation of miR-509-3p expression by collagen type XI alpha 1-regulated hypermethylation facilitates cancer progression and chemoresistance via the DNA methyltransferase 1/Small ubiquitin-like modifier-3 axis in ovarian cancer cells.
H Tu et al. Am J Cancer Res 2023; 13 (6):2307-2322 Loss of MEG3 contributes to the enhanced migration and invasion in arsenic-induced carcinogenesis through NQO1/FSCN1 pathway
Wu YH et al. Res Sq 2023; The Downregulation of miR-509-3p Expression by Collagen Type XI Alpha 1-Regulated Hypermethylation Facilitates Cancer Progression and Chemoresistance via the DNA Methyltransferase 1/Small Ubiquitin-like Modifier-3 Axis in Ovarian Cancer Cells.
Xu J et al. Cancers (Basel) 2022; 14 (17) DNMT3A/<i>miR-129-2-5p</i>/Rac1 Is an Effector Pathway for <i>SNHG1</i> to Drive Stem-Cell-like and Invasive Behaviors of Advanced Bladder Cancer Cells.
Shirui Huang et al. Cancer Cell Int 2021; 21 (1):241 miR-190 promotes malignant transformation and?progression of human urothelial cells through CDKN1B/p27 inhibition.
Application : WB
Reactivity : Human
Camero S et al. Cells 2021; 10 (11) DNMT3A and DNMT3B Targeting as an Effective Radiosensitizing Strategy in Embryonal Rhabdomyosarcoma.
Application : WB
Reactivity : Human
Williamson SM et al. J Insect Physiol 2021; 134:104298 Epigenetic underpinnings of freeze avoidance in the goldenrod gall moth, Epiblema scudderiana.
Application : WB
Tessier SN et al. Mol Cell Biochem 2021; 476 (11):3975-3985 Epigenetic regulation by DNA methyltransferases during torpor in the thirteen-lined ground squirrel Ictidomys tridecemlineatus.
Yan JB et al. Mol Ther Oncolytics 2020; 18:282-294
Li J et al. Cell Discov 2020; 6:58 USP7 negatively controls global DNA methylation by attenuating ubiquitinated histone-dependent DNMT1 recruitment.
Application : WB
Reactivity : Human
Liao CH et al. Mar Drugs 2019; 17 (9) Epigenetic Modification and Differentiation Induction of Malignant Glioma Cells by Oligo-Fucoidan.
Application : WB
Reactivity : Human
Wei GJ et al. AAPS J 2019; 21 (5):86 A Tangeretin Derivative Inhibits the Growth of Human Prostate Cancer LNCaP Cells by Epigenetically Restoring p21 Gene Expression and Inhibiting Cancer Stem-like Cell Proliferation.
Application : WB
Reactivity : Human
Huang MH et al. Pharmacol Res 2018; 139:50-61 Epigenetic targeting DNMT1 of pancreatic ductal adenocarcinoma using interstitial control release biodegrading polymer reduced tumor growth through hedgehog pathway inhibition.
Application : WB
Reactivity : Human
Weng YP et al. Oncotarget 2018; 9 (1):361-374 The inhibitory activity of gallic acid against DNA methylation: application of gallic acid on epigenetic therapy of human cancers.
Application : WB
Reactivity : Human
Shih CC et al. PLoS One 2016; 11 (9):e0163690 Procainamide Inhibits DNA Methylation and Alleviates Multiple Organ Dysfunction in Rats with Endotoxic Shock.
Application : WB
Reactivity : Rat
Wijenayake S et al. J Comp Physiol B 2016; 186 (3):333-42 The role of DNA methylation during anoxia tolerance in a freshwater turtle (Trachemys scripta elegans).
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169