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DOCK2 antibody

Anti-DOCK2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130734
Anti-DOCK2 antibody used in Western Blot (WB). GTX130734

Cat. No. GTX130734

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

212 kDa. ( Note )

Positive Control

Jurkat , Raji , NCI-H929

Predict Reactivity

Mouse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.49 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human DOCK2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886333

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

dedicator of cytokinesis 2 , IMD40

Cellular Localization

Endomembrane system , Cytoplasm , cytoskeleton

Background

The DOCK2 gene encodes a hematopoietic cell-specific CDM family protein that is indispensable for lymphocyte chemotaxis.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-DOCK2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130734

GTX130734 ICC/IF Image

DOCK2 antibody detects DOCK2 protein at cytoplasm by immunofluorescent analysis.
Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DOCK2 protein stained by DOCK2 antibody (GTX130734) diluted at 1:400.
Blue: Hoechst 33342 staining.

Anti-DOCK2 antibody used in Western Blot (WB). GTX130734

GTX130734 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DOCK2 antibody (GTX130734) diluted at 1:1000.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169