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NUMB antibody

Anti-NUMB antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30689
Anti-NUMB antibody used in Western Blot (WB). GTX30689
Anti-NUMB antibody used in Western Blot (WB). GTX30689

Cat. No. GTX30689

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat, Chicken
Package
100 μl ($419)
See all NUMB products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.2 - 0.5 μg/ml
ICC/IF 1:500
Not tested in other applications.

Calculated MW

71 kDa. ( Note )

Product Note

This is specific for all four isoforms of the NUMB protein.

PROPERTIES

Form

Liquid

Buffer

Tris-Glycine, 0.15M NaCl

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

3.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

A synthetic peptide made to a C-terminal region of mouse NUMB (between residues 600-653). [UniProt# Q9QZS3]

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NUMB endocytic adaptor protein , Nb

Background

Plays a role in the process of neurogenesis. Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate. Not required for the proliferation of neural progenitor cells before the onset of neurogenesis. Also involved postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity. May also mediate local repair of brain ventricular wall damage.

Database

Research Area

DATA IMAGES

Anti-NUMB antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30689

GTX30689 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX30689 NUMB antibody.
Green : primary antibody
Red : Tubulin
Blue : DAPI
Dilution : 1:500

Anti-NUMB antibody used in Western Blot (WB). GTX30689

GTX30689 WB Image

WB analysis of A431 cell lysate using GTX30689 NUMB antibody.

Anti-NUMB antibody used in Western Blot (WB). GTX30689

GTX30689 WB Image

WB analysis of A431 cell lysate using GTX30689 NUMB antibody.
Dilution : 0.5 μg/ml
Loading : 20μg

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 419