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PC2 antibody

Anti-PC2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23533
Anti-PC2 antibody used in Western Blot (WB). GTX23533
Anti-PC2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23533

Cat. No. GTX23533

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all PC2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 2 μg/ml
ICC/IF 1:20-1:200
Not tested in other applications.

Calculated MW

71 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Synthetic peptide corresponding to residues E(622) L D E A V E R S L Q S I L R K N(638) of mouse PC2.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_385097

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

proprotein convertase subtilisin/kexin type 2 , 6330411F23Rik , AI839700 , Nec-2 , Nec2 , PC2 , Phpp-2 , SPC2

Cellular Localization

Secreted

Background

The subtilisin-like prohormone convertase (PC) family is a group of cellular enzymes that cleave most prohormones and neuropeptide precursors. Numerous other cellular proteins, some viral proteins, and bacterial toxins that are transported by the constitutive secretory pathway are also targeted for maturation by PCs. PC family members share structural similarities, which include a heterogeneous ~10 kDa amino-terminal proregion, a highly conserved ~55 kDa subtilisin-like catalytic domain, and carboxyl-terminal domain that is heterogeneous in length and sequence. These enzymes become catalytically active following proregion cleavage within the appropriate cellular compartment. The subcellular localization of PC family members varies. Immunolocalization studies show that PC1 is found in the perinuclear region as well as the trans-Golgi network, whereas PC2 can be found in the trans-Golgi network as well as diffusely distributed in the peripheral cytoplasm.

Database

Research Area

DATA IMAGES

Anti-PC2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23533

GTX23533 ICC/IF Image

ICC/IF analysis of C2C12 cells using GTX23533 PC2 antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 incubated overnight at 4 ºC

Anti-PC2 antibody used in Western Blot (WB). GTX23533

GTX23533 WB Image

WB analysis of 25 ug of mouse cerebellum cell lysates using GTX23533 PC2 antibody.
Dilution : 1:500

Anti-PC2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23533

GTX23533 ICC/IF Image

ICC/IF analysis of HEK293 cells using GTX23533 PC2 antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 incubated overnight at 4 ºC

REFERENCE

Application Reference

Neus Barranco et al. Transl Neurodegener 2021; 10 (1) : 37 Dense core vesicle markers in CSF and cortical tissues of patients with Alzheimer's disease.
Knoch KP et al. Mol Metab 2014; 3 (5) : 518-30 ptbp1 is required for glucose-stimulated cap-independent translation of * granule proteins and coxsackieviruses in beta cells.
Application : WB
Reactivity : Mouse
Hatanaka M et al. Hum Mol Genet 2011; 20 (7) : 1274-84 Wolfram syndrome 1 gene (WFS1) product localizes to secretory granules and determines granule acidification in pancreatic beta-cells.
Application : WB
Reactivity : Mouse
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399