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PPAR delta antibody

Anti-PPAR delta antibody used in Immunohistochemistry (IHC). GTX28937
Anti-PPAR delta antibody used in Western Blot (WB). GTX28937

Cat. No. GTX28937

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ELISA, IHC

Reactivity

 Mouse, Rat
Package
50 μg ($399)
See all PPAR delta products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:5000
ELISA 1:30000 - 1:70000
IHC Assay dependent
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.02M Potassium Phosphate, 0.15M NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Synthetic peptide: MEQPQEETPEAREE (Mouse).

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

peroxisome proliferator activator receptor delta , NUC-1 , NUC1 , Nr1c2 , PPAR-beta , PPAR-delta , PPAR[b] , PPARdelta , Pparb , Pparb/d

Cellular Localization

Nucleus

Background

The Peroxisome Proliferator-Activated Receptors (PPARs) are members of the nuclear hormone receptor subfamily of transcription factors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. There are 3 known subtypes of PPARs, PPAR-alpha, PPAR-delta and PPAR-gamma. PPAR-gamma is believed to be involved in adipocyte differentiation.

Database

Research Area

DATA IMAGES

Anti-PPAR delta antibody used in Immunohistochemistry (IHC). GTX28937

GTX28937 IHC Image

Rabbit Anti-PPAR delta (N terminal specific) 1:500

Anti-PPAR delta antibody used in Western Blot (WB). GTX28937

GTX28937 WB Image

Western blotting. Affinity Purified Anti-PPAR delta (Rabbit) is shown to detect a predominant band at ~ 43 kDa corresponding to PPAR delta present in mouse heart whole cell lysates. Preincubation of the antibody with the immunizing peptide completely blocks reactivity with this band (data not shown). Approximately 30 μg of lysate was loaded per lane for SDS-PAGE. Detection occurred using a 1:750 dilution of primary antibody diluted in 5% BLOTTO in PBS overnight at 4º C followed reaction with a 1:10,000 dilution of IRDyeR 800 conjugated goat anti-Rabbit IgG (H&L) for 45 min at room temperature (800 nm channel, green). IRDyeR 800 fluorescence image was captured using the OdysseyR Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399