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eIF4E antibody

Anti-eIF4E antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50553
Anti-eIF4E antibody used in IHC (Paraffin sections) (IHC-P). GTX50553
Anti-eIF4E antibody used in Western Blot (WB). GTX50553

Cat. No. GTX50553

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399)
See all eIF4E products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around aa. 207~211 (S-G-S-T-T) derived from human eIF4E.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11172204

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

eukaryotic translation initiation factor 4E , AUTS19 , CBP , EIF4E1 , EIF4EL1 , EIF4F , eIF-4E

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of messenger RNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the 5'-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. This gene acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. Several pseudogenes of this gene are found on other chromosomes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-eIF4E antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50553

GTX50553 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50553 eIF4E antibody.

Anti-eIF4E antibody used in IHC (Paraffin sections) (IHC-P). GTX50553

GTX50553 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50553 eIF4E antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

Anti-eIF4E antibody used in Western Blot (WB). GTX50553

GTX50553 WB Image

WB analysis of extracts from HeLa, HepG2, and 293 cells using GTX50553 eIF4E antibody.

REFERENCE

Application Reference

Uniacke J et al. Cancer Res 2014; Cancer Cells Exploit eIF4E2-Directed Synthesis of Hypoxia Response Proteins to Drive Tumor Progression.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399