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SNAP23 antibody

Anti-SNAP23 antibody used in Western Blot (WB). GTX23340
Anti-SNAP23 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23340
Anti-SNAP23 antibody used in Western Blot (WB). GTX23340

Cat. No. GTX23340

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, FACS, IP

Reactivity

 Human, Mouse, Rat, Bovine
Package
100 μg ($399)
See all SNAP23 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-2 μg/ml
ICC/IF 2 μg/ml
FACS 3-5 μg/10⁶ cells
IP Assay dependent
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Sythetic Peptide: C N(193) K N R I D I A N T R A K K L I D S(210)

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

synaptosomal-associated protein 23 , 23kDa , AA408749 , SNAP-23 , Sndt , Syndet

Cellular Localization

Cell membrane

Background

Synaptosomal-associated proteins (SNAPs) are cytosolic proteins that play a key role in in the process of membrane fusion in intracellular vesicle trafficking. In eukaryotic cells, the SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) complex is critical to membrane docking and fusion and is believed to impart some degree of specificity between vesicle SNARE (v-SNARE) and target organelle SNARE (t-SNARE). In neurons and neuroendocrine cells, the SNARE complex consists of the integral membrane proteins VAMP (vesicle-associated membrane protein), syntaxin and SNAP-25. In non-neuronal tissue, a SNAP-25 homolog, SNAP-23, functionally replaces SNAP-25 in the SNARE complex. Studies show that VAMP, syntaxin and SNAP-23 are required for SNARE function and that this complex exists as a heterotrimer of the three proteins. In insulin signalling pathways, studies suggest that the translocation of GLUT4 in adipocytes requires that functional SNAP-23 be present in SNARE complexes.

Database

Research Area

DATA IMAGES

Anti-SNAP23 antibody used in Western Blot (WB). GTX23340

GTX23340 WB Image

WB analysis of rat brain protein extract using GTX23340 SNAP23 antibody.

Anti-SNAP23 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23340

GTX23340 ICC/IF Image

ICC/IF analysis of U-87 MG cells using GTX23340 SNAP23 antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 2 μg/ml in 0.1% BSA incubated for 3 hours at room temperature

Anti-SNAP23 antibody used in Western Blot (WB). GTX23340

GTX23340 WB Image

WB analysis of membrane enriched extracts (30 μg lysate) of U-87 MG (Lane 1) and Hep G2 (Lane 2) using GTX23340 SNAP23 antibody.
Dilution : 2 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399