Application Note
For IP: Use at an assay dependent dilution. For WB: Use at a concentration of 2 -3 μg/ml. Predicted molecular weight: 59.7 kDa. GTX16885 may appear as a doublet on WB due to phosphorylation. This antibody can be used to precipitate active src kinase. Not suitable for IF, IHC-Fr or IHC-P. Optimal dilutions/concentrations should be determined by the researcher.
Calculated MW
Positive Control
COLO-201 cells
Product Note
Reacts with both v-Src and c-Src proteins.
Form
Liquid
Buffer
50mM Sodium Phosphate buffer pH7.4, 0.2% Gelatin
Preservative
0.1% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
Concentration
0.1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Mouse
Immunogen
Antibody generated by immunizing BALB/c mice with full length native protein (purified) and then fusing with P3X63 Ag8.653 myeloma cells.
Purification
Purified IgG
From hybridoma culture supernatant
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Synonyms
Rous sarcoma oncogene , AW259666 , pp60c-src
Cellular Localization
Cell membrane , Mitochondrion inner membrane , Nucleus , Cytoplasm, cytoskeleton
Database
Research Area