APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| Assay dependent |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Predict Reactivity
Bovine, Pig, Xenopus tropicalis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.81 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human ACSL3. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10720304
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
acyl-CoA synthetase long chain family member 3 , ACS3 , FACL3 , LACS 3 , LACS3 , PRO2194
Cellular Localization
Mitochondrion outer membrane; Single-pass type III membrane protein , Peroxisome membrane; Single-pass type III membrane protein , Microsome membrane; Single-pass type III membrane protein , Endoplasmic reticulum membrane; Single-pass type III membrane pr
Background
The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme is highly expressed in brain, and preferentially utilizes myristate, arachidonate, and eicosapentaenoate as substrates. The amino acid sequence of this isozyme is 92% identical to that of rat homolog. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX112431 IHC-P Image
ACSL3 antibody detects ACSL3 protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
ACSL3 stained by ACSL3 antibody (GTX112431) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX112431 IHC-P Image
ACSL3 antibody detects ACSL3 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.ACSL3 stained by ACSL3 antibody (GTX112431) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX112431 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human breast cancer, using ACSL3(GTX112431) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX112431 ICC/IF Image
ACSL3 antibody detects ACSL3 protein at endoplasmic reticulum by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: ACSL3 stained by ACSL3 antibody (GTX112431) diluted at 1:500.
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GTX112431 IHC-P Image
ACSL3 antibody detects ACSL3 protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
ACSL3 stained by ACSL3 antibody (GTX112431) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX112431 IHC-P Image
ACSL3 antibody detects ACSL3 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
ACSL3 stained by ACSL3 antibody (GTX112431) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX112431 IHC-P Image
ACSL3 antibody detects ACSL3 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
ACSL3 stained by ACSL3 antibody (GTX112431) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX112431 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACSL3 antibody (GTX112431) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX112431 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACSL3 antibody (GTX112431) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
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