GeneTex

  • Country / Location Selection

United States (US)

ABCB7 antibody [C1C3]

Anti-ABCB7 antibody [C1C3] used in Western Blot (WB). GTX114916
Anti-ABCB7 antibody [C1C3] used in Western Blot (WB). GTX114916

Cat. No. GTX114916

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse, Drosophila
Package
100 μl ($399),
25 μl ($169)
See all ABCB7 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ABCB7. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619260

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATP binding cassette subfamily B member 7 , ABC7 , ASAT , Atm1p , EST140535

Cellular Localization

Mitochondrion inner membrane

Background

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance as well as antigen presentation. This gene encodes a half-transporter involved in the transport of heme from the mitochondria to the cytosol. With iron/sulfur cluster precursors as its substrates, this protein may play a role in metal homeostasis. Mutations in this gene have been implicated in X-linked sideroblastic anemia with ataxia. [provided by RefSeq]

Database

  • Gene ID: 22 ABCB7
  • UniProt: O75027 ABCB7

Research Area

DATA IMAGES

Anti-ABCB7 antibody [C1C3] used in Western Blot (WB). GTX114916

GTX114916 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ABCB7 antibody [C1C3] (GTX114916) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ABCB7 antibody [C1C3] used in Western Blot (WB). GTX114916

GTX114916 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ABCB7 antibody [C1C3] (GTX114916) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

Application Reference

Piel RB 3rd et al. Biochemistry 2016; 55 (37) : 5204-17 A Novel Role for Progesterone Receptor Membrane Component 1 (PGRMC1): A Partner and Regulator of Ferrochelatase.
Application : WB
Reactivity : Human
Medlock AE et al. PLoS One 2015; 10 (8) : e0135896 Identification of the Mitochondrial Heme Metabolism Complex.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

There are currently no reviews for ABCB7 antibody [C1C3] (GTX114916). Be the first to share your experience with this product.
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169