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TRPC4 antibody

Anti-TRPC4 antibody used in IHC (Frozen sections) (IHC-Fr). GTX54758
Anti-TRPC4 antibody used in Western Blot (WB). GTX54758
Anti-TRPC4 antibody used in Immunoprecipitation (IP). GTX54758
Anti-TRPC4 antibody used in Western Blot (WB). GTX54758

Cat. No. GTX54758

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IP

Reactivity

 Human, Mouse, Rat
Package
50 μl ($639)
See all TRPC4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

112 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.4 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Peptide (C)KEKHAHEEDSSIDYDL, corresponding to amino acid residues 943-958 (Intracellular, C-terminus) of mouse TRPC4 (Accession Q9QUQ5).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887882

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

transient receptor potential cation channel, subfamily C, member 4 , CCE1 , STRPC4 , Trp4 , Trrp4

Cellular Localization

Membrane,Cell membrane

Database

Research Area

DATA IMAGES

Anti-TRPC4 antibody used in IHC (Frozen sections) (IHC-Fr). GTX54758

GTX54758 IHC-Fr Image

IHC-Fr analysis of mouse cerebellum tissue using GTX54758 TRPC4 antibody.
Panel A : TRPC4 (red) appears in Purkinje cells (arrows) and in the molecular (Mol) layer.
Panel B : Staining with mouse anti-parvalbumin (PV)  in the same brain section.
Panel C : Merge of TRPC4 and PV demonstrates partial co-localization in the Purkinje and the molecular layers.

Anti-TRPC4 antibody used in Western Blot (WB). GTX54758

GTX54758 WB Image

WB analysis of PC3 (lanes 1,3) and LNCaP cell lysates using GTX54758 TRPC4 antibody preincubated with or without immunogen peptide.
Dilution : 1:200

Anti-TRPC4 antibody used in Immunoprecipitation (IP). GTX54758

GTX54758 IP Image

IP analysis of rat brain tissue lysate using GTX54758 TRPC4 antibody. The upper arrow indicates TRPC4 channel while the lower arrow indicates the IgG heavy chain. WB was performed with the GTX54758 antibody.
IP reaction : 6 μg
Lane 1 : Rat brain lysate
Lane 2 :  Lysate immunoprecipitated with  Anti-TRPC4  Antibody
Lane 3 : Lysate immunoprecipitated with pre-immune rabbit serum

Anti-TRPC4 antibody used in Western Blot (WB). GTX54758

GTX54758 WB Image

WB analysis of rat brain membrane lysate using GTX54758 TRPC4 antibody preincubated with or without immunogen peptide.
Dilution : 1:200

REFERENCE

Application Reference

Hi Lu et al. Am J Transl Res 2018; 10 (8) : 2290-2305 Entresto therapy effectively protects heart and lung against transverse aortic constriction induced cardiopulmonary syndrome injury in rat.
Application : IHC-P
Reactivity : Rat
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 639