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FAM171A1 antibody [C1C3]

Orthogonal
Anti-FAM171A1 antibody [C1C3] used in Western Blot (WB). GTX120226
Anti-FAM171A1 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX120226
Anti-FAM171A1 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX120226
Anti-FAM171A1 antibody [C1C3] used in Western Blot (WB). GTX120226

Cat. No. GTX120226

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all FAM171A1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

98 kDa. ( Note )

Positive Control

HepG2 , HCT116

Predict Reactivity

Mouse, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Intracellular domain of human FAM171A1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10720303

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

family with sequence similarity 171 member A1 , APCN , C10orf38

Cellular Localization

Membrane; Single-pass type I membrane protein

Database

Research Area

DATA IMAGES

Anti-FAM171A1 antibody [C1C3] used in Western Blot (WB). GTX120226

GTX120226 WB Image

Sample (30 ug of whole cell lysate)
A: HepG2
B: HCT116
7.5% SDS PAGE
GTX120226 diluted at 1:1000

Anti-FAM171A1 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX120226

GTX120226 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using FAM171A1(GTX120226) antibody at 1:500 dilution.

Anti-FAM171A1 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX120226

GTX120226 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using FAM171A1(GTX120226) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-FAM171A1 antibody [C1C3] used in Western Blot (WB). GTX120226

GTX120226 WB Image

Orthogonal

The data was published in the journal Cell Death Dis in 2019. PMID: 31649243

REFERENCE

Application Reference

Sanawar R et al. Oncogenesis 2019; 8 (1) : 5 Estrogen Receptor-α Regulation of microRNA-590 Targets FAM171A1-a Modifier of Breast Cancer Invasiveness.
Bao C et al. Cell Death Dis 2019; 10 (11) : 807 Exploring specific prognostic biomarkers in triple-negative breast cancer.
Application : WB
Reactivity : Human
Zhang D et al. Nature 2019; 574 (7779) : 575-580 Metabolic regulation of gene expression by histone lactylation.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169