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ABCD2 antibody [N3C2], Internal

Comparison Orthogonal
Anti-ABCD2 antibody [N3C2], Internal used in Western Blot (WB). GTX112623
Anti-ABCD2 antibody [N3C2], Internal used in Western Blot (WB). GTX112623

Cat. No. GTX112623

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all ABCD2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

Positive Control

SHSY5Y

Predict Reactivity

Mouse, Rat, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.77 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ABCD2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949555

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATP binding cassette subfamily D member 2 , ABC39 , ALDL1 , ALDR , ALDRP , hALDR

Cellular Localization

Peroxisome membrane

Background

The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ALD subfamily, which is involved in peroxisomal import of fatty acids and/or fatty acyl-CoAs in the organelle. All known peroxisomal ABC transporters are half transporters which require a partner half transporter molecule to form a functional homodimeric or heterodimeric transporter. The function of this peroxisomal membrane protein is unknown; however this protein is speculated to function as a dimerization partner of ABCD1 and/or other peroxisomal ABC transporters. Mutations in this gene have been observed in patients with adrenoleukodystrophy, a severe demyelinating disease. This gene has been identified as a candidate for a modifier gene, accounting for the extreme variation among adrenoleukodystrophy phenotypes. This gene is also a candidate for a complement group of Zellweger syndrome, a genetically heterogeneous disorder of peroxisomal biogenesis. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ABCD2 antibody [N3C2], Internal used in Western Blot (WB). GTX112623

GTX112623 WB Image

ABCD2 antibody detects ABCD2 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ABCD2 antibody (GTX112623) diluted by 1:500.

Anti-ABCD2 antibody [N3C2], Internal used in Western Blot (WB). GTX112623

GTX112623 WB Image

Comparison Orthogonal

SH-SY5Y whole cell and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with ABCD2 antibody [N3C2], Internal (GTX112623) diluted at 1:500 and competitor's antibody (18179-1-AP) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

REFERENCE

Application Reference

Chornyi S et al. J Lipid Res 2023; 64 (5) : 100364 The origin of long-chain fatty acids required for de novo ether lipid/plasmalogen synthesis.
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169