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TRIM32 antibody

Overexpression
Anti-TRIM32 antibody used in IHC (Paraffin sections) (IHC-P). GTX113937
Anti-TRIM32 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113937
Anti-TRIM32 antibody used in Western Blot (WB). GTX113937
Anti-TRIM32 antibody used in Western Blot (WB). GTX113937

Cat. No. GTX113937

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all TRIM32 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

72 kDa. ( Note )

Positive Control

DDDDK-human TRIM32-transfected 293T cells

Predict Reactivity

Rat, Bovine, Cat, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TRIM32. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2038168

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tripartite motif containing 32 , BBS11 , HT2A , LGMD2H , LGMDR8 , TATIP

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies. The protein has also been localized to the nucleus, where it interacts with the activation domain of the HIV-1 Tat protein. The Tat protein activates transcription of HIV-1 genes. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TRIM32 antibody used in IHC (Paraffin sections) (IHC-P). GTX113937

GTX113937 IHC-P Image

TRIM32 antibody detects TRIM32 protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
TRIM32 antibody (GTX113937) diluted at 1:400.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TRIM32 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113937

GTX113937 ICC/IF Image

TRIM32 antibody detects TRIM32 protein at cytoplasm by immunofluorescent analysis.
Sample: A375 cells were fixed in ice-cold MeOH for 5 min.
Green: TRIM32 protein stained by TRIM32 antibody (GTX113937) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-TRIM32 antibody used in Western Blot (WB). GTX113937

GTX113937 WB Image

TRIM32 antibody detects TRIM32 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
7.5% SDS-PAGE
TRIM32 antibody (GTX113937) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TRIM32 antibody used in Western Blot (WB). GTX113937

GTX113937 WB Image

Overexpression

TRIM32 antibody detects TRIM32 protein by western blot analysis.
A. 5 μg 293T whole cell lysate/extract
B. 5 μg whole cell lysate/extract of DDDDK-human TRIM32-transfected 293T cells
7.5% SDS-PAGE
TRIM32 antibody (GTX113937) dilution: 1:20000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Prajapati P et al. Cell Signal 2020; 76 : 109777 TRIM32 regulates mitochondrial mediated ROS levels and sensitizes the oxidative stress induced cell death.
Application : WB
Reactivity : Human
Wang M et al. Cell Death Differ 2019; Trim32 suppresses cerebellar development and tumorigenesis by degrading Gli1/sonic hedgehog signaling.
Application : WB
Reactivity : Mouse
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169