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FBXO21 antibody

Anti-FBXO21 antibody used in Western Blot (WB). GTX121415
Anti-FBXO21 antibody used in Western Blot (WB). GTX121415

Cat. No. GTX121415

Reference 1
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all FBXO21 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IP Assay dependent
Not tested in other applications.

Calculated MW

72 kDa. ( Note )

Positive Control

U87-MG , Rat-2

Predict Reactivity

Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.95 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human FBXO21. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10731589

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

F-box protein 21 , FBX21

Background

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-FBXO21 antibody used in Western Blot (WB). GTX121415

GTX121415 WB Image

FBXO21 antibody detects FBXO21 protein by Western blot analysis.
A. 30 μg Rat-2 whole cell lysate/extract
7.5 % SDS-PAGE
FBXO21 antibody (GTX121415) dilution: 1:1000

Anti-FBXO21 antibody used in Western Blot (WB). GTX121415

GTX121415 WB Image

Sample (30 ug of whole cell lysate)
A: U87-MG
7.5% SDS PAGE
GTX121415 diluted at 1:2000

REFERENCE

Application Reference

Yoshida Y et al. Proc Natl Acad Sci U S A 2015; 112 (15) : 997234 A comprehensive method for detecting ubiquitinated substrates using TR-TUBE.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

FBXO21 antibody Cat. No. GTX121415

Rating
( Average 4 based on 1 users reviews)
Application
Immunoprecipitation(IP)
( Average 4 based on 1 users reviews)
Anti-FBXO21 antibody used in Immunoprecipitation (IP). GTX121415
Date : Anonymous submitted on 01-Jan-2000
Score :
Application Tested : IP
Sample Species : Ms
Sample : mouse FBXO21 transfected HEK293T cells
Amount used : 20μg
Blocking : 2% skim milk/ 0.5% BSA in PBS/0.01% Tween 20, 4°C, 2Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Lane Description : A:Mock transfection - IP with flag, B:Flag-tagged FBXO21 -IP flag, C:Flag tagged FBXO21- IP with GTX121415, D:Fermentag pageruler plus prestained marker, E:Input control IP A, F:Input control IP B, G:Input control IP C, H:Total lysate mock transfected HEK 293T
Figure Note : 2.375 ug for IP
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169