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GGA3 antibody [C3], C-term

Anti-GGA3 antibody [C3], C-term used in Western Blot (WB). GTX106461
Anti-GGA3 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX106461
Anti-GGA3 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX106461
Anti-GGA3 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106461

Cat. No. GTX106461

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all GGA3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

78 kDa. ( Note )

Positive Control

A431

Predict Reactivity

Rat, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.59 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human GGA3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950375

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

golgi associated, gamma adaptin ear containing, ARF binding protein 3

Cellular Localization

Golgi apparatus , trans-Golgi network membrane; Peripheral membrane protein , Endosome membrane; Peripheral membrane protein

Background

This gene encodes a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) family. This family includes ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins. Alternative splicing of this gene results in two transcript variants. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GGA3 antibody [C3], C-term used in Western Blot (WB). GTX106461

GTX106461 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
7.5% SDS PAGE
GTX106461 diluted at 1:2000

Anti-GGA3 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX106461

GTX106461 IHC-P Image

GGA3 antibody [C3], C-term detects GGA3 protein at cytoplasm on mouse stomach by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
GGA3 antibody [C3], C-term (GTX106461) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GGA3 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX106461

GTX106461 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using GGA3(GTX106461) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GGA3 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106461

GTX106461 ICC/IF Image

GGA3 antibody [C3], C-term detects GGA3 protein at Golgi apparatus by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: GGA3 protein stained by GGA3 antibody [C3], C-term (GTX106461) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169