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SEPT8 antibody

Anti-SEPT8 antibody used in Western Blot (WB). GTX119118
Anti-SEPT8 antibody used in IHC (Paraffin sections) (IHC-P). GTX119118
Anti-SEPT8 antibody used in Western Blot (WB). GTX119118
Anti-SEPT8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119118
Anti-SEPT8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119118

Cat. No. GTX119118

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all SEPT8 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

HCT116 , mouse brain , rat brain

Predict Reactivity

Rabbit, Bovine, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.83 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SEPT8. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617374

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

septin 8 , SEP2

Background

SEPT8 is a member of the highly conserved septin family. Septins are 40- to 60-kD GTPases that assemble as filamentous scaffolds. They are involved in the organization of submembranous structures, in neuronal polarity, and in vesicle trafficking (Blaser et al., 2003 [PubMed 12909369]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-SEPT8 antibody used in Western Blot (WB). GTX119118

GTX119118 WB Image

Various tissue extracts (10 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SEPT8 antibody (GTX119118) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SEPT8 antibody used in IHC (Paraffin sections) (IHC-P). GTX119118

GTX119118 IHC-P Image

SEPT8 antibody detects SEPT8 protein at cytosol on U87 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U87 xenograft.
SEPT8 antibody (GTX119118) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SEPT8 antibody used in Western Blot (WB). GTX119118

GTX119118 WB Image

Sample (30 ug of whole cell lysate)
A: HCT116
12% SDS PAGE
GTX119118 diluted at 1:2000

Anti-SEPT8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119118

GTX119118 ICC/IF Image

SEPT8 antibody detects SEPT8 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SEPT8 protein stained by SEPT8 antibody (GTX119118) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-SEPT8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119118

GTX119118 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using 39332(GTX119118) antibody (Green) at 1:200 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169